Lecture
Near-infrared optical nanosensors for real-time cell analytics, bioanalytics and biotechnological sensing
- at -
- A3.527
- Type: Lecture
Lecture description
Real-time, label-free monitoring of cellular and microbial states is a key requirement in modern cell analytics, bioanalytics and biotechnological process control. Here, we present a concept for near-infrared (NIR) optical nanosensors based on single-walled carbon nanotubes (SWCNTs) that can be integrated as thin sensor coatings into a wide range of in vitro formats, including standard cell culture labware, imaging chambers, slides and microfluidic devices. The nanosensors emit in the NIR biological transparency window and combine high photostability with exceptional sensitivity to biomolecules. Through tailored surface functionalization, highly specific biosensors can be created for metabolites, neurotransmitters and stress-associated signaling molecules, providing a dynamic “biochemical fingerprint” of cellular or microbial state. Immobilized as coatings, the SWCNT nanosensors convert conventional substrates into optically active biointerfaces that are compatible with established cell culture, live-cell imaging and screening workflows. The NIR optical nanosensor concept opens versatile application spaces, including real-time monitoring of drug responses and cellular signaling dynamics, analysis of metabolic and stress responses, bioreactor monitoring and the development of future rapid diagnostic and point-of-care assays.
