Analysis of Anionic and Cationic Polar Pesticides Using a New Mixed Mode Column.
Javier Arrebola1, Roberto Romero Gonzalez1, Antonia Garrido1, Marta Vargas1, Richard Jack2, Ramkumar Dhandapani2, Zara Jalali2
1Department of Chemistry and Physics, University of Almeria, Carr. Sacramento, s/n, 04120 La Canada, Almeria, Spain
2Phenomenex, Inc., Torrance, CA
Analysis of polar pesticides presents multiple challenges including adequate retention, separation of critical pairs, and reproducibility. Current HPLC columns available is dedicated to specific mode of analysis, can require long equilibration and hence results in low throughput. In addition, food matrices can add additional challenges due to the presence of complex matrix components including pigments, fats, and sugars that can interfere with the analyte of interest. polar, anionic analytes like Glyphosate will utilize QuEChERS or QuPPE sample preparation techniques, followed by HILIC LC-MS/MS methods for chromatographic retention and separation. Historically, these methods are not user friendly, and lack reproducibility necessary for a commercial laboratory application. In this study, we are presenting a unique HPLC selectivity that provides optimal separation of various anionic pesticides including Glyphosate, Chlorate, Perchlorate, Ethephon, Phosphoric acid-based pesticides, and N-Ac-Glucosamine plus chlorate and perchlorate. In addition, we demonstrate optimal separation and detection of various cationic polar pesticides in both reverse phase and HILIC modes followed by MS detection. Thus both anionic and cationic pesticides can be analyzed in one, reversed phase separation due to the mixed mode capabilities of this unique column. The study demonstrates robust polar pesticide analysis from real sample matrix in addition to providing fast equilibration, optimal retention and peak symmetry for both anionic and cationic pesticides in various food extracts. This column has recently been published in QuPPe-PO v12.2.
