Lecture
Tracing the progress of analytical strategies for detection of amatoxins in human biosamples
- at -
- ICM Saal 5
- Type: Lecture
Lecture description
Amatoxin-containing mushrooms cause severe intoxications and fatalities each year. Amatoxins are thermally stable bicyclic octapeptides that primarily damage the liver and kidneys, potentially leading to multi-organ failure and death. Due to the delayed onset of severe clinical symptoms, timely diagnosis relies on rapid, sensitive, and reliable detection, which is crucial for effective treatment and improved patient outcomes. Over the past decades, analytical approaches have been established, beginning with the Meixner-Wieland test, the first colorimetric assay for the detection of amatoxin-containing mushrooms. Nowadays, immunoassays, including the radioimmunoassay, enzyme-linked immunosorbent assay, or lateral flow immunoassay, are being utilized to determine the presence of amatoxins in biosamples, usually urine. However, given their limited sensitivity and specificity, liquid-chromatography (LC)-mass spectrometry (MS) has become the preferred analytical technique, combining selectivity and separation efficiency with increased detectability [1]. Prior to LC-MS analysis, appropriate sample preparation is important, as amatoxins are often present only in trace amounts. Extraction can be performed either by conventional techniques, including protein precipitation, liquid-liquid extraction, and solid-phase extraction, or via antibody-based immunoaffinity enrichment [1-4]. This talk will focus on analytical strategies for detecting amatoxins in human biosamples, tracing the progress from early approaches to recent advancements.
1. Barbosa, I., et al., Analytical methods for amatoxins: A comprehensive review. J Pharm Biomed Anal, 2023. 232: p. 115421.
2. Bambauer, T.P., et al., Development and application of a strategy for analyzing eight biomarkers in human urine to verify toxic mushroom or ricinus communis ingestions by means of hydrophilic interaction LC coupled to HRMS/MS. Talanta, 2020. 213: p. 120847.
3. Vollmer, A.C., et al., Rapid analysis of amatoxins in human urine by means of affinity column chromatography and liquid chromatography-high-resolution tandem mass spectrometry. Sci Rep, 2024. 14(1): p. 21397.
4. Maurer, H.H., et al., Validated electrospray liquid chromatographic-mass spectrometric assay for the determination of the mushroom toxins alpha- and beta-amanitin in urine after immunoaffinity extraction. J Chromatogr B Biomed Sci Appl, 2000. 748(1): p. 125-35.
1. Barbosa, I., et al., Analytical methods for amatoxins: A comprehensive review. J Pharm Biomed Anal, 2023. 232: p. 115421.
2. Bambauer, T.P., et al., Development and application of a strategy for analyzing eight biomarkers in human urine to verify toxic mushroom or ricinus communis ingestions by means of hydrophilic interaction LC coupled to HRMS/MS. Talanta, 2020. 213: p. 120847.
3. Vollmer, A.C., et al., Rapid analysis of amatoxins in human urine by means of affinity column chromatography and liquid chromatography-high-resolution tandem mass spectrometry. Sci Rep, 2024. 14(1): p. 21397.
4. Maurer, H.H., et al., Validated electrospray liquid chromatographic-mass spectrometric assay for the determination of the mushroom toxins alpha- and beta-amanitin in urine after immunoaffinity extraction. J Chromatogr B Biomed Sci Appl, 2000. 748(1): p. 125-35.
