Lecture
The impact of ion mobility spectrometry in the general screening and quantification of drugs and metabolites in plasma and urine
- at -
- ICM Saal 5
- Type: Lecture
Lecture description
M. Cifuentes Girard, L. Ekmekciu, P. Mueller and G. Hopfgartner
For the general screening of endogenous and exogenous compounds, in biological samples, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) becomes the method of choice. However, the technique still suffers from drawbacks
such as limited structural information, moderate sample throughput or incomplete isomer separation of drugs and metabolites. Ion mobility spectrometry (IMS) enables the separation of charge particles based on their charge and shape and various forms
of IMS have been described such a drift time IMS (DTIMS) or Differential mobility spectrometry (DMS) for the separation of isomeric compounds. In DMS ions are exposed to high- and low-field conditions and the addition of a modifier in the carrier gas (e.g.) methanol, toluene,…) is straightforward. DMS can be considered as a tunable filter, based on chemical selectivity. and can be coupled either to triple quadrupoles or high-resolution mass spectrometers and offers an additional separation dimension to LC and MS. DMS can play has an analytical game changer and this additional separation dimension can be used to reduce analysis time in quantification¹ or for comprehensive multiple dimension separation LCxDMSXMS/MS². IMS separation is not limited to even electron ions formed by electrospray but also works with radical cation precursor generated by dopant assisted atmospheric pressure photoionization (dAPPI).
For the general screening of endogenous and exogenous compounds, in biological samples, liquid chromatography coupled with tandem mass spectrometry (LC-MS/MS) becomes the method of choice. However, the technique still suffers from drawbacks
such as limited structural information, moderate sample throughput or incomplete isomer separation of drugs and metabolites. Ion mobility spectrometry (IMS) enables the separation of charge particles based on their charge and shape and various forms
of IMS have been described such a drift time IMS (DTIMS) or Differential mobility spectrometry (DMS) for the separation of isomeric compounds. In DMS ions are exposed to high- and low-field conditions and the addition of a modifier in the carrier gas (e.g.) methanol, toluene,…) is straightforward. DMS can be considered as a tunable filter, based on chemical selectivity. and can be coupled either to triple quadrupoles or high-resolution mass spectrometers and offers an additional separation dimension to LC and MS. DMS can play has an analytical game changer and this additional separation dimension can be used to reduce analysis time in quantification¹ or for comprehensive multiple dimension separation LCxDMSXMS/MS². IMS separation is not limited to even electron ions formed by electrospray but also works with radical cation precursor generated by dopant assisted atmospheric pressure photoionization (dAPPI).
Furthermore, acoustic ejection mass spectrometry (AEMS) combined with open port probe (OPP) is emerging as an ultrahigh-throughput approach, allowing the analysis of hundreds of samples in less than a few minutes with very low sample consumption.
To overcome the lack of chromatography, DMS can bring additional separation selectivity at an MS time scale of a few milliseconds for the screening of Drug of Abuse.
1) Cifuentes Girard, M. F.; Knight, P.; Hopfgartner, G. Drug Test Anal 2024. DOI: 10.1002/dta.3778
2) Ekmekciu, L.; Hopfgartner, G. Anal. Bioanal. Chem. 2023. DOI: 10.1007/s00216-023-04602-0.
