Lecture
Semi-permeable capsules a novel tool for high-throughput single-cell -omics
- at -
- ICM Saal 2
- Type: Lecture
Lecture description
Analysis of heterogeneous biological systems increasingly relies on high-throughput methods and tools that efficiently probe the cellular phenotype and genotype. While recent advancements have enabled various single-cell -omics assays, their broader
applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. I will present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of highthroughput nucleic acid assays, including digital PCR, single-cell RNA- and DNAsequencing (scRNA-Seq and scDNA-Seq) and FACS-based sorting of individual transcriptomes based on any nucleic acid marker of interest. Being biocompatible, the
SPCs support single-cell cultivation and clonal expansion over long periods of time – a fundamental limitation of droplet microfluidics systems. Using SPCs we perform scRNA-Seq on white blood cells from patients with hematopoietic disorders and demonstrate that SPCs offer superior transcript capture, even for the most challenging cell types. Accurate representation of entire cell diversity of clinical samples, new insights into the malfunctioning of the innate immune system within disease context
and ability to clonally expand individual cells over long period of time, positions SPC technology as sensitive and broadly applicable tool for easy-to-use, scalable singlecell -omics assays.
applications are inherently limited by the challenge of efficiently conducting multi-step biochemical assays while retaining various biological analytes. I will present a versatile technology based on semi-permeable capsules (SPCs), tailored for a variety of highthroughput nucleic acid assays, including digital PCR, single-cell RNA- and DNAsequencing (scRNA-Seq and scDNA-Seq) and FACS-based sorting of individual transcriptomes based on any nucleic acid marker of interest. Being biocompatible, the
SPCs support single-cell cultivation and clonal expansion over long periods of time – a fundamental limitation of droplet microfluidics systems. Using SPCs we perform scRNA-Seq on white blood cells from patients with hematopoietic disorders and demonstrate that SPCs offer superior transcript capture, even for the most challenging cell types. Accurate representation of entire cell diversity of clinical samples, new insights into the malfunctioning of the innate immune system within disease context
and ability to clonally expand individual cells over long period of time, positions SPC technology as sensitive and broadly applicable tool for easy-to-use, scalable singlecell -omics assays.
