Lecture
Immunophenotyping for Accurate Classification of Leukemias and Lymphomas
- at -
- ICM Saal 4a
- Type: Lecture
Lecture description
Flow cytometry is an integral part of multidisciplinary diagnostics of hematologic neoplasms in combination with cytomorpholgy, histopathology, chromosome banding anaylsis, fluorescence in situ hybridization and various molecular genetic methods including PCR, quantitative PCR, NGS as well as WTS and WGS. Flow cytometry is used in combination with cytomorphology to rapidly confirm or rule out the suspected disease and to specifically guide the following genetic analyses. Flow cytometry takes advantage of identifying antigen expression pattern specific of typical for various hematologic neoplasms and thereby differentiating respective cell populations from normal and reactive ones. It applies multiple parameters with cutting edge routine application on the way from
8-10 to 13-15 simultaneously detected fluorescence dyes thus providing comprehensive characterization of cell populations. Further technical improvement is anticipated by the introduction of spectral cytometry allowing for doubling of the number of simultaneously detected fluorescence dyes. Besides diagnostics flow cytometry is used for sensitively monitoring of measurable residual disease (MRD) in acute leukemias as well as in CLL and multiple myeloma. The presentation will cover the respective aspects of flow cytometry.
8-10 to 13-15 simultaneously detected fluorescence dyes thus providing comprehensive characterization of cell populations. Further technical improvement is anticipated by the introduction of spectral cytometry allowing for doubling of the number of simultaneously detected fluorescence dyes. Besides diagnostics flow cytometry is used for sensitively monitoring of measurable residual disease (MRD) in acute leukemias as well as in CLL and multiple myeloma. The presentation will cover the respective aspects of flow cytometry.
