Harmonisation of ion mobility-mass spectrometry data with new biological reference materials
- at -
- ICM Saal 2
- Type: Lecture
Lecture description
(1) BOKU University, Institute of Analytical Chemistry, Department of Natural Sciences and Sustainable Resources
(2) BOKU University, Institute of Microbiology and Microbial Biotechnology, Department of Biotechnology and Food Science
Ion mbility-mass spectrometry (IM-MS) is an advanced analytical technique combining mass spectrometry with gas-phase separation based on ion mobility, allowing ions to be separated not only by their mass-to-charge ratio (m/z), but also by their size, charge, and conformation. IM-MS provides deeper chemical and structural information in fields such as proteomics, metabolomics, lipidomics, structural biology and environmental analysis. Additionally, one of the key properties derived from these measurements is the IM-derived collision cross section (CCS), which is widely touted as a new identification parameter for a range of application areas.
Despite the obvious potential of IM-MS, there are major challenges for users due to the lack of standardisation and harmonisation for determining CCS [1]. At present, there are no certified reference materials for routine measurements with IM-MS, which significantly limits use of CCS data especially considering the range of different instrument types available. To try to overcome this challenge, we are developing inexpensive and broadly applicable candidate reference materials suited for three classes of compounds (metabolites, lipids and peptides) from a yeast known for its high biomass yields and concentrations (Pichia pastoris, or more commonly referred to as
Komagataella phaffii) [2].
Building on the excellent interlaboratory reproducibility achieved with commercial drift tube IM-MS [3], this technology will be used as the benchmark for development of measurement and reporting standards of all materials. Subsequently, materials will be extensively characterised and tested as part of the MobiliTraIN project [4], allowing the widest range of instrument types (e.g., traveling wave, trapped ion mobility, structures for lossless ion manipulation) and applications to be considered. The coordination of the required stability, homogeneity, and interlaboratory testing is supported by collaboration with the Federal Institute for Materials Research and Testing (BAM)
providing the pathway to the certification of materials for routine measurement and reporting of CCS data with IM-MS.
Literature:
[1] V. Gabelica, Mass Spectrom. Rev. 2019, 38, 291-320.
[2] L. Heistinger, Microbiology 2020, 166, 614-6167.
[3] S.M. Stow, Anal. Chem. 2017, 89, 9048-9055.
[4] https://mobilitrain.eu/
