Lecture
Clinical lipidomics: Stability of lipids in blood samples
- at -
- ICM Saal 2
- Type: Lecture
Lecture description
Clinical lipidomics investigations are one of the current cornerstones to elucidate either prognostic and diagnostic biomarkers, or new therapeutic targets, or discover and understand pathophysiological mechanisms. Hence clinical lipidomics is a commonly applied analytical profiling tool for research. But time and again, scientists find that the results obtained are disappointing and completely different from what they expected. The reason for this is often unclear. One possibility that is less considered, but nevertheless not uncommon, is the fact that in whole blood lipids remain stable in concentration for very different lengths of time after blood collection. After separating the plasma from the cellular components of blood, the stability of most lipids is significantly improved. This means that any deviation, whether accidental or systematic, in the handling of whole blood between the collection site in the hospital and the sample processing location (e.g. delays in transportation, exposure to ambient temperature) can lead to changes in certain lipid concentrations and consequently the results of the lipidomics study.
This presentation will demonstrate which lipid species are very stable in whole blood and suitable for profiling in everyday clinical blood samples. On the other hand, recommendations will also be discussed to avoid changes to less stable lipids in such approaches. Furthermore, unpublished data on the stability of lipids in a special lipidomics strategy for studying bioactive lipids in whole blood will be presented.
This presentation will demonstrate which lipid species are very stable in whole blood and suitable for profiling in everyday clinical blood samples. On the other hand, recommendations will also be discussed to avoid changes to less stable lipids in such approaches. Furthermore, unpublished data on the stability of lipids in a special lipidomics strategy for studying bioactive lipids in whole blood will be presented.
