Lecture
Advanced and automated sample preparation by sublimation for high spatial resolution MALDI Imaging on the timsTOF fleX
- at -
- ICM Saal 2
- Type: Lecture
Lecture description
Single‑cell mass spectrometry imaging demands high spatial resolution, sensitivity, and consistently reproducible sample preparation. These requirements are often difficult to meet due to analyte delocalization, heterogeneous crystal formation, and insufficient control over matrix deposition. To address these challenges, we evaluated a MALDI imaging workflow that integrates automated sublimation and optional recrystallization to improve matrix uniformity and spatial fidelity.
The workflow uses predefined sublimation parameters that stabilize crystal morphology and deposition homogeneity, reducing operator‑dependent variability. In combination with microgrid‑based high‑resolution sample preparation at 5 µm raster width, this approach maintains analyte localization and enhances comparability across individual cells and biological replicates.
Data acquired from samples prepared with this workflow and analyzed on a timsTOF fleX MALDI‑2 system show improved spatial definition, reduced chemical background, and more reliable molecular annotations at cellular resolution. Results from multiple laboratories further demonstrate the robustness of the preparation strategy across a range of tissue types.
Overall, automated sublimation and controlled recrystallization represent effective measures for increasing reproducibility and data quality in single‑cell MALDI imaging, supporting more reliable and scalable spatial‑omics investigations.
The workflow uses predefined sublimation parameters that stabilize crystal morphology and deposition homogeneity, reducing operator‑dependent variability. In combination with microgrid‑based high‑resolution sample preparation at 5 µm raster width, this approach maintains analyte localization and enhances comparability across individual cells and biological replicates.
Data acquired from samples prepared with this workflow and analyzed on a timsTOF fleX MALDI‑2 system show improved spatial definition, reduced chemical background, and more reliable molecular annotations at cellular resolution. Results from multiple laboratories further demonstrate the robustness of the preparation strategy across a range of tissue types.
Overall, automated sublimation and controlled recrystallization represent effective measures for increasing reproducibility and data quality in single‑cell MALDI imaging, supporting more reliable and scalable spatial‑omics investigations.
